Used LIFE TECHNOLOGIES iBind Western Device

The iBind Flex Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind Flex device wells to an iBind Flex Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Manufacture Specification

• Simple, automated solution

• No external power source

• Superior reproducibility over traditional manual methods

• Better antigen antibody interaction—solutions flow between the iBind Card and membrane for maximum interaction between protein and antibody